BioDiamond One tube RT-PCR kit 50rx 反轉錄混合酵素套組
No:DMDRT003
Pack Size:50rx
Description
The 2x One-tube RT-PCR Hot Start kit is designed for combining two reactions of reverse transcription and PCR. It provides more simple and effective operation in RT-PCR. The reverse transcription step is working by M-MLV RT (H-). The PCR reaction is working by Hot start Taq DNA Polymerase. An especially reaction buffer is provided both for Reverse Transcriptase and Hot Start Taq DNA Polymerase.
Storage condition
-20°C
Mix component M-MLV RTase
Hot start Taq
RT-PCR reaction buffer
dNTP
stabilizer
One tube RT-PCR result
The 2x One-tube RT-PCR Hot Start kit is designed for combining two reactions of reverse transcription and PCR. It provides more simple and effective operation in RT-PCR. The reverse transcription step is working by M-MLV RT (H-). The PCR reaction is working by Hot start Taq DNA Polymerase. An especially reaction buffer is provided both for Reverse Transcriptase and Hot Start Taq DNA Polymerase.
Storage condition
-20°C
Mix component M-MLV RTase
Hot start Taq
RT-PCR reaction buffer
dNTP
stabilizer
One tube RT-PCR result
Sensitivity
Targets can generally be detected from < 1 pg to 50 ng polyA RNA(mRNA) or 10 pg to 1µg total RNA. Even lower amounts of RNA may be successfully amplified by using highly expressed transcripts.
RT-PCR cycles program
Targets can generally be detected from < 1 pg to 50 ng polyA RNA(mRNA) or 10 pg to 1µg total RNA. Even lower amounts of RNA may be successfully amplified by using highly expressed transcripts.
RT-PCR cycles program
Analyzing the RT-PCR Products
Analyze the RT-PCR products by 1.0% (w/v) agarose gel electrophoresis. The products will be visible by UV transillumination of the ethidium bromide-stained agarose gel. Store the reaction products at –20°C until needed.
Analyze the RT-PCR products by 1.0% (w/v) agarose gel electrophoresis. The products will be visible by UV transillumination of the ethidium bromide-stained agarose gel. Store the reaction products at –20°C until needed.
20181226_dmdrt003_one_tube_rt-pcr_kit50rx.pdf | |
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