WB 5X Bradford reagent kit
No: F1BRA500
Size: 500 ml
Store at 2~8 ℃
Product information:
For protein determination (1:4 dilution)
Bradford assay is the most popular assays for protein determination methods, which is based on the color switch of brilliant blue G-250 after binding to proteins in acidic condition. WB 5X Bradford Reagent Kit is modified with general Bradford assay, however, have more enhanced sensitivity, stability, and perfect color change in your experiment. Compare to other commercial Bradford assays, WB 5X Bradford Reagent Kit have concentrated features, lower unnecessary biohazard materials such as methanol or other toxic solvents, and still have good performance!
Protocol:
Standard assay
- Preparation of BSA standard, 2 mg/ml.
- Prepare three to five serial dilutions of BSA standard or unknown samples to 1.5 ml tube.
- Add 800 ml of each standard and sample into each 1.5 ml tube.
- Add 200 ml of WB 5X Bradford Reagent Kit to each tube, mix well, and transfer to cuvette or microplate.
- read absorbance at 595 nm.
High sensitive microwell assay
- Preparation of BSA standard, 2 mg/ml.
- Add 0.25 to 2 ml of BSA standard or unknown samples to each well .
- Prepare 1x WB 5X Bradford Reagent Kit working dye solution by mix 1 part of WB 5X Bradford Reagent Kit with 4 part ultra-pure water.
- Add 200ml of 1x WB 5X Bradford Reagent Kit working dye solution in each well.
- Mix welled, and read absorbance at 595 nm.