WB Protease Inhibitor Cocktail (EDTA-Free, Twenty-five mini-Tablets)
No: F1PICo25
Size: 25 mini tablets
Store at 4 ℃ for 2 years
.Description :
Endogenous proteins are produced and removed in a balanced state,so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradationis enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize acocktail of small molecule inhibitors to block the action of proteases. The cocktail functions to inhibit proteases that would degrade eitherphosphorylated or non-phosphorylated protein substrates. WB Protease Inhibitor Cocktail (EDTA free, Twenty-five mini-Tablets) is a blend of 5 pan-protease inhibitors for protection of protein integrity. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 actsagainst cystein proteases. Leupeptin acts against both serine and cystein proteases.
.Protocol :
(1) This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
(2) One mini-Tablet can be added to 10 mL extraction medium directly. Alternatively, a stock solution (10X conc. one mini-Tablet in 1 mL ddH2O) can be prepared. Vortex briefly to accelerate the dissolution. The stock solution is stable for one week when stored at 4°C, and for at least 4 weeks at -20°C.
.Components :