WB Quickly Prestained Protein Ladder 250ul 蛋白質標記
Cat No. F1PP180250-2
Size: 2X 250 μl
Store at -20 °C
Description
The WB Prestained Protein Ladder is a prestained mixture of ten recombinant proteins ranging from 10 kDa to 180 kDa. Three different chromophores are bound to the proteins, producing a brightly colored ladder. The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional reducing agent necessary. Lot-to-lot variation of the apparent molecular weight of prestained proteins is ~5%.
Storage Buffer
62.5mM Tris•H3PO4 (pH 7.5 at 25°C),
1mM EDTA, 2% (w/v) SDS,
10mM DTT, 1mM NaN3,
33% (v/v) glycerol.
Recommendations for Loading
1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on an SDS-polyacrylamide gel:
• 5 μl per well for mini gel, 10 μl per well for large gel.
• Use the same volumes for Western blotting.
• The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm.
The loading volume should be doubled for 1.5 mm thick gels.