WB Quickly ECL reagent kit
No: F1ECL200
Size: 100ml + 100ml
No: F1ECL500
Size: 250ml + 250ml
Store at 2~8 ℃
This product is for laboratory research ONLY and not for diagnostic use.
Description
The principle of WB ECL Reagent is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blotting,Southern and Northern. HRP catalyze the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by hydrogen peroxide (H2O2).
WB ECL Reagent can enhance the luminol-dependent chemiluminescence and can be wildly use to detect the present of HRP-conjugated antibodies or
streptavidin which binding to antigen or nucleotide sequence respectively.
Storage
WB ECL Reagent is stable for2~8°C
Procedure
1.Mix the solution A 1:1 with solution B in WB ECL Reagent and incubate the mixture for 1 min in room temperature.
2.Add the sufficient mixture solution to cover the membrane (0.1ml/cm2).
Incubate the membrane for 1 min in room temperature.
3.Discard the excess mixture in membrane and wrap the membrane in saran wrap. Carefully and gently remove the air bubbles from the membrane.
4.Place the membrane in the film cassette and keep the protein side up. Turn off
the lights and use safety light. Then place a sheet of film on the membrane and close the cassette and expose for 10-90 seconds.
5.Open cassette and transfer the exposed film to developing machine. Then place a new film on the membrane and expose again.
6.The exposure time of second film can be adjusted by the intensity of first film.
7.If the intensity was too high, please wait up to 10 minutes before re-exposing.
Note
1. Keep the membrane in wet after first antibody incubation.
2. Keep the LumiFast Long Chemiluminescence Detection Kit mixture fresh and do not reuse the LumiFast Long Chemiluminescence Detection mixture.
3. Please use new tip to avoid contamination.
4. Step 1-3 soown in procedural can not be handled in darkroom.
5. The antibody on the membrane can be remove by using BioFU Western Blot Stripping Reagent (JB11-K002) and reprobe again.
Description
The principle of WB ECL Reagent is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blotting,Southern and Northern. HRP catalyze the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol by hydrogen peroxide (H2O2).
WB ECL Reagent can enhance the luminol-dependent chemiluminescence and can be wildly use to detect the present of HRP-conjugated antibodies or
streptavidin which binding to antigen or nucleotide sequence respectively.
Storage
WB ECL Reagent is stable for2~8°C
Procedure
1.Mix the solution A 1:1 with solution B in WB ECL Reagent and incubate the mixture for 1 min in room temperature.
2.Add the sufficient mixture solution to cover the membrane (0.1ml/cm2).
Incubate the membrane for 1 min in room temperature.
3.Discard the excess mixture in membrane and wrap the membrane in saran wrap. Carefully and gently remove the air bubbles from the membrane.
4.Place the membrane in the film cassette and keep the protein side up. Turn off
the lights and use safety light. Then place a sheet of film on the membrane and close the cassette and expose for 10-90 seconds.
5.Open cassette and transfer the exposed film to developing machine. Then place a new film on the membrane and expose again.
6.The exposure time of second film can be adjusted by the intensity of first film.
7.If the intensity was too high, please wait up to 10 minutes before re-exposing.
Note
1. Keep the membrane in wet after first antibody incubation.
2. Keep the LumiFast Long Chemiluminescence Detection Kit mixture fresh and do not reuse the LumiFast Long Chemiluminescence Detection mixture.
3. Please use new tip to avoid contamination.
4. Step 1-3 soown in procedural can not be handled in darkroom.
5. The antibody on the membrane can be remove by using BioFU Western Blot Stripping Reagent (JB11-K002) and reprobe again.