BioDiamond M-MLV reverse transcriptase
No:Dr.QRT001
Size: 10000 U
- Description
- Storage conditions:-20°C
- Unit description
- Supplied 5xRT buffer
MgCl 2
DTT
- Storage buffer
100 mM NaCl
1 mM EDTA
0.1 mM DTT
0.1% Triton X-100
50% glycerol
- Two step RT-PCR result
200 units of enzyme are used to produce cDNA from 1µg of a 1.2kb control RNA. The minimum specification is 120ng of first strand cDNA made from 1µg of RNA. The cDNA product must be >90% full length.
- Contaminant Activity
for one hour at 37°C. Following incubation, the supercoiled DNA is visualized on an ethidium bromide- stained agarose gel to verify the absence of visible nicking or cutting (analysis on 0.4µg of DNA).
- Physical Purity
Dr.Q M-MLV reverse transcriptase 10000u(dr.qrt001)說明書.pdf | |
File Size: | 333 kb |
File Type: |