WB Quickly Run buffer 10X
No:F1RUN500
Size:500 ml
Store at 2~8 ℃
Protocol
Prepare a 1X running buffer Store at 4C solution by diluting the stock buffer with distilled water.
1. Fill your electrophoresis chamber.
2. Install your gel(s), flush the wells with running buffer and load your samples.
3. We recommend, when you test this buffer for the first time, to load a prestained
MW marker in the marker lane to see the migration length and stop it as you need.
4. Run under your prefered conditions. Please be careful, the migration is faster then
your regular buffer.
As a guide, we recommend the following conditions :
Tris/SDS/ PAGE Protocol:
170V, for about 35 min.
Gradient Gel Protocol:
1.Stacking 100V, 5 min
2.Gradient Gel 170V 35 min.