WB Quickly Rapid gel stain
No:F1RAP500
Size: 500 ml
Store at RT
Product information:
For protein staining (1:6 dilution)
Compare to other traditional Coomassie briilient blue staining, this product have more sensitivity, more quickly to performing the staining procedure, getting more clear background, without have methanol and acetic acid, and can be easy destained by H2O. Therefore saving your time, money, and getting better results.
Protocol:
In SDS-PAGE:
- After electrophoresis, wash SDS-PAGE twice with distilled H2O for 5 minutes.
- Dilute 7x WB 5X Rapid Gel Stain to 1x with ultrapure H2O.
- Put the gel into Diluted WB 5X Rapid Gel Stain and shake it for 15-30 minutes to 1 hours. Normally, the several protein bands or spots will become visible after 5minutes under this step.
- Destained: Discard the residual WB 5X Rapid Gel Stain, wash the gel with distilled H2O till the background become clear. The dye bounded proteins will become more visible during the wash step. Put the gel in ddH2O for overnight that can get more clearly results never worry about the dye-protein binding affinity.
In PVDF membrane:
- After electrophoresis, wash PVDF twice with 95% methanol for 5 minutes.
- Let methanol washed PVDF completed dry.
- Dilute 7x WB 5X Rapid Gel Stain to 1x with ultrapure H2O.
- Put the dried PVDF into Diluted WB 5X Rapid Gel Stain and shake it for 15-30 minutes to 1 hours. Normally, the several protein bands or spots will become visible after 5minutes under this step.
- Destained: Discard the residual WB 5X Rapid Gel Stain, wash the gel with 30-35% ethanol till the background become clear.
- Wash the PVDF with distilled H2O. The dye bounded proteins will become more visible during the H2O wash step.